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Next, 100 L of supernatant was transferred to a solution consisting of 1200 L of 0.12 M sodium phosphate buffer, pH 8.0, 100 L of 0.1 M N-ethylmaleimide (NEM, Sigma-Aldrich, Darmstadt, Germany), and 50 L of nicotinamide adenine dinucleotide reduced disodium salt hydrate (NADH, Sigma-Aldrich, Darmstadt, Germany)

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[79], which compared metformin, clomifene citrate, and pioglitazone, but did not include emerging drug therapies such as GLP-1 RAs and SGLT2is

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